狠狠色丁香久久综合频道,无码人妻丰满熟妇区毛片,亚洲国产欧美在线人网站,亚洲欧美日韩国产一级A

歡迎進(jìn)入上海起發(fā)實(shí)驗(yàn)試劑有限公司!
產(chǎn)品展示

Nucleosome antigen

描述:Identity:Core histones (H2A, H2B, H3, H4) and DNA (approximay 146 bp)Source Material:Bovine thymus (New Zealand origin).Clinical Indications:Autoantibodies present in systemic lupus erythematosus a

更新時(shí)間:2016-11-09
訪問(wèn)次數(shù):2679
廠商性質(zhì):代理商
詳情介紹

NUCLEOSOME ANTIGEN 
AROTEC_Nucleosome_Product_Info.pdf Version/Date: A/00.08.18 
ATN02-02 Nucleosome antigen 0.20 mg 
ATN02-05 Nucleosome antigen 0.50 mg 
ATN02-10 1.0 mg 
_________________________________________________________________________________
Description of the Product
Purified from bovine thymus. After coating onto ELISA plates 
the product will bind autoantibodies to nucleosome antigen. 
Purity: The nucleosome antigen is more than 90% pure, as 
assessed by SDS gel electrophoresis. 
Concentration: 0.5 -2.0 mg protein/ml. 
Storage: The product is stabilised with 0.1% Micr-O-protectTM

Store at -20 o
C or below (long term) or at +4o
C (short term). 
Avoid repeated freezing and thawing. Mix thoroughly before 
use. 
Clinical and Biochemical Data 
Anti-nucleosome antibodies (also referred to as anti-chromatin 
antibodies) constitute one of the first autoantibody specificities 
found to be present in systemic lupus erythematosus (SLE) 
having been detected as early as 1948 using the LE cell 
test1,2. The prevalence of anti-nucleosome antibodies in SLE is 
of the order of 50-100%2
, and the nucleosome has been 
identified as the initiating and driving immunogen in SLE3,4

Antinucleosome antibodies are now considered to be a more 
sensitive marker for SLE than anti-dsDNA antibodies5
 and a 
high anti-nucleosome antibody titre has been reported to be 
indicative of lupus nephritis6-8. Anti-nucleosome antibodies 
have been found (with a lower prevalence than in SLE) in a 
number of other autoimmune diseases6,9,10,19 such as systemic 
sclerosis, Sjogren's syndrome and mixed connective tissue 
disease and are also found in 40-50% of patients with 
autoimmune hepatitis type I11,12. Anti-ribosomal P antibodies 
have also been reported to bind to nucleosomes13,14

The nucleosome is the basic structural subunit of chromatin, 
the native complex of histones and DNA found in the nucleus 
of eukaryotic cells. It is composed of about 200 base pairs of 
DNA wrapped twice around a (H2A-H2B-H3-H4)2 histone 
octamer with histone H1 bound on the periphery2,15,16,19

Nucleosomes can be isolated by digesting the linker DNA 
holding them together in chromatin with micrococcal nuclease. 
The most effective form of nucleosomes for detecting patient 
autoantibodies are prepared in this way and stripped of H1 
histone to yield a nucleosome core particle in which 146 base 
pairs of DNA are wrapped around an octamer of two H2A-H2B 
dimers that surround an H3-H4 tetramer2,17. Autoantibodies 
against subnucleosome particles occur in SLE, they are 
however less frequent3

AROTEC nucleosome antigen is prepared from calf thymus 
chromatin and contains the four core histones H2A, H2B, H3 
and H4 bound to DNA of about 146 base pairs in length. 
Histone amino acid sequences are highly conserved between 
species, even between animals and plants18. Homology 
between human and bovine amino acid sequences is as 
follows (ExPASy accession numbers human:bovine are 
shown): 
H2A (P0C0S5:P0C0S4) 128 aa: 128 identical 
H2B (B2R4S9:A5D7N2) 126 aa: 125 identical, 1 conservative 
subsitition 
H3 (P84243:Q5E9F8) 136 aa: 136 identical 
H4 (P62805:P62803) 103 aa: 103 identical 
Methodology
The following is an ELISA procedure which can be used to 
detect anti-nucleosome autoantibodies in human serum using 
the ATN02 purified nucleosome antigen: 
1. Dilute the purified antigen to 1.0-2.0 ?g/ml in 20 mM 
Tris/HCl buffer, pH 8.0; containing 0.15 M NaCl. 
2. Coat ELISA plates with 100 ?l of diluted antigen per well. 
Cover and incubate 24 hours at +4o
C. 
3. Empty the plates and remove excess liquid by tapping on a 
paper towel. 
4. Block excess protein binding sites by adding 200 ?l PBS 
containing 1% BSA per well. Cover and incubate at +4o

overnight. 
5. Empty plates and apply 100 ?l of serum samples diluted 
1:100 in PBS / 1% BSA / 0.1% Tween?
 20. Incubate at room 
temperature for 1 hour. 
6. Empty plates and add 200 ?l PBS / 0.1% Tween?
 20 per 
well. Incubate 5 minutes then empty plates. Repeat this step 
twice. 
7. Apply 100 ?l anti-human IgG-enzyme conjugate 
(horseradish peroxidase or alkaline phosphatase) diluted in 
PBS / 1% BSA / 0.1% Tween?
 20 per well and incubate for 1 
hour. 
8. Repeat step 6. 
9. Add enzyme substrate and stop the reaction when 
appropriate. 
10. Read absorbance in an ELISA spectrophotometer. 
References 
1. Hargraves, M.M. et al. (1948) Proc. Mayo Clin. 23, 25 
2. Gomez-Puerta, J.A. et al. (2008) Autoimmunity Rev. 7, 606 
3. Burlingame, R.W. et al. (1994) J. Clin. Invest. 94, 184 
4. Muller, S. et al. (2008) Lupus 17, 431 
5. Putova, I et al. (2007) Annals N.Y. Acad. Sci. 1109, 275 
6. Cervera, R. et al. (2003) Ann. Rheum. Dis. 62, 431 
7. Manson, J.J. (2009) Arthritis Res. Ther. 11, R154 
8. Kiss, E. et al. (2009) Autoimmunity 42, 393 
9. Amoura, Z. et al. (2000) Arthritis Rheum. 43, 76 
10. Schett, G. et al. (2002) Lupus 11, 704 
11. Ghillani-Dalbin, P. et al. (2003) Lupus 12, 833 
12. Koutouzov, S. et al. (2004) Rheum. Dis. Clin. North Am. 30, 529 
13. Chindalore, V. et al. (1998) Clin. Immunol. Immunopathol. 87, 292 
14. Caponi, L. et al. (2002) Clin. Exp. Immunol. 130, 541 
15. Lutter, L.C. (1978) J. Mol. Biol. 124, 391 
16. Luger, K. et al. (1997) Nature 389, 251 
17. Burlingame, R.W. & Rubin, R.L. (1990) J. Immunol. Meth. 134, 187 
18. Baxevanis, A.D. & Landsman, D. (1996) Nucleic Acid Res. 24, 245 
19. Decker, P. (2006) Clin. Chim. Acta 366, 48 
Micr-O-protect is from Roche Diagnostics GmbH (Mannheim, 
Germany). 
Tween?
 20 is a registered trademark of ICI Americas Inc. 
NOTE: No patented technology has been used by AROTEC 
during the preparation of this product. 

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
上一條:La (SSB) antigen
下一條:PCNA antigen
亚洲一区二区三区视频下载| 红杏无吗一区区三区四区| 欧美 日韩国产综合在线| 少妇高潮尖叫久久久久久久| 精品久久久久久久无码| 少妇无码ay无码专区线| 午夜影院免费在线观看电影| 2021年国产精品久久| 日韩视频一区二区三区视频| www亚洲中文在线日本| 国产成人免费A在线视频| av电影手机版在线观看| 在线观看一本到欧美日韩| 鸡巴操烂小骚逼高清无码| 国产精品亚洲精品日韩精品| 精品秘一区二区三区四区| 精品久久久久久中文字幕无码软件| 很污很黄的网站在线观看| 三上悠亚av作品在线观看| 少妇激情αv一区二区三区| 天天影视色香欲综合视频| 国产高清一区二区更新区| 国产精品99久久久久久a| 波多野结衣电影在线观看| 动漫大鸡吧成人在线观看| 久久精品久久久久久免费| 欧美一区二区三区狠狠色| 三级网站网址久久久久久| 色欲国产麻豆一精品一av一免费| 亚洲天堂视频网| 亚洲综合色婷婷中文字幕| 日韩免费一级无码av片| 国内亚洲欧美一区二区三区| 国产激情在线播放你懂的| 日本中文字幕有码在线看| 国产精品久久久久久久人妻| 无码丰满熟妇一区二区浪| 色哟哟欧美一区二区三区| 亚洲久久久视频在线观看| 性做久久久久久久| 久久久久久久久久免费剧|